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Bacterial and Fungal Study of 100 Cases of Chronic Suppurative Otitis Media.
Authore(s) : Dr.R.Usha Rani
Volume : (3), Issue : 211, January - 2019
Abstract : Objective – To study the bacterial and fungal organisms present in chronic suppurative otitis media and to study the antibiotic sensitivity pattern of the bacterial isolates.Methodology – A total number of 100 patients with clinical diagnosis of CSOM, attending the ENT OP comprised the study group. Three pus swabs from each patient discharging ear were collected. One pus swab is used for smear preparation, second swab for bacterial cultures and third swab for fungal cultures.Bacterial isolates identified and antibiotic susceptibility testing is done.Fungal isolates were identified by conventional methods.The findings were analysed.Results – Out of 100 patients, 53% were females and 47% were males. In this 40% belonged to 0-15 yrs age group. From 100 bacterial cultures 95 bacterial strains were isolated. In this Pseudomonas aeruginosa ( 36 ), Klebsiella(20),Staphylococcus aureus(10) followed by other organisms. Differ- ent fungal organisms isolated from 100 cultures were Candida albicans(12),Aspergillus flavus(5), Aspergillus niger(4). Study of antibiotic susceptibility of 95 bacterial isolates revealed that Imipenem is most effective without resistance followed by Amikacin(6.3% resistance), Ge ntamicin(7.3%),Ciprofloxicin(10.5%) followed by other antibiotics.Conclusion – The present study stresses the need for careful isolation of both bacterial and fungal organisms to establish the role of fungi in CSOM pathology and to prevent administration of unwanted antibiotics. INTRODUCTION CSOM with its complications is one of the common diseases in the practice of otologist, paediatrician and general practitioner. CSOM is an infection of the middle ear and mastoid cavity 1 .It is defined as persistent or intermittent infected discharge of more than 3 months duration through perforated or non intact tympanic membrane caused by bacteria, fungi and viruses re- sulting in inflammation of the mucosal lining that often results in partial or total loss of tympanic membrane and the ossicles 5 . CSOM is one of the most common causes of deafness and can cause permanent perforation of tympanic membrane. World Health Organization had estimated that CSOM prevalence in In- dia, Tanzania, Soloman islands, Guam, Australian aborigins and Greenland is highest(> 4%) and urgent attention is needed to deal with the health problem 1 . In the recent years a steady in- crease in the incidence of gram negative bacterial infections has been observed. Many a times random antibiotic therapy prior to a defined laboratory diagnosis, leads to indiscriminate use of an- tibiotics, which results in emergence of resistant strains. Recent advances in the management of CSOM emphasize the necessity of bacteriological examination of ear discharge and mycological study.In the present study an earnest attempt was made to study the bacterial and fungal flora of CSOM cases. METHODOLOGY A total number of 100 patients with clinical diagnosis of CSOM attending ENT OP department of Govt. General Hospital, Kaki- nada during February 2012 to September 2014, comprised the study group. All these are not having recent treatment with an- tibiotics either locally or systemically. These cases included are individuals of both sexes and all age groups.Clinical evaluation of the disease was done by presence of per- foration of tympanic membrane and otorrhoea which are the two presenting symptoms of patients. The discharging pus was collected under aseptic conditions with the help of sterile swabs in triplicate by dipping into deep meatus through it drains. The pus samples from each patient was analyzed in the department of Microbiology.The material of the first swab was used for making smears and for KOH preparation for microscopic examination. The second swab was used
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Article: Download PDF Journal DOI : 2364/2018
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Bacterial isolates, Fungal isolates, Antibiotic susceptibility.
Vol.I (3), Issue.I 211