
Development of Nano particles with Tetracycline with Interfacial Deposition of PHB (Poly-Β-Hydroxybutyrate)
Authore(s) : Mekala M || Assistant professorDepartment of MicrobiologySri Ramakrishna College of Arts and Science for WomenCoimbatore-641 014Tamil NaduIndia.
Volume : (3), Issue : 211, January - 2019

Abstract : Different soil samples were collected for the isolation of PHB producing bacillus sp present in and around Coimbatore area. As the preliminary analysis the screening, extraction and estimation of PHB, Physical optimization of PHB production, chemical characterization of extracted powder was done by FTIR analysis. Tetracycline is the drug of choice for per oral administration using nanoprecipitation technique. The production of Poly-β-hydroxybutyrate (PHB) as nanoparticle containing Tetracycline, increase the stability of loaded drug. The extracted PHB created as nanoparticles and the nano encapsulation was done by PCL immobilization method with presence and absence of drug. The resulting nanoparticle is characterized by Scanning Electron Microscopy (SEM) analysis.
Keywords :Extraction of PHB, Nano encapsulation, Nanoparticles, Interfacial deposition Tetracycline.
Article: Download PDF Journal DOI : 2364/2018
Cite This Article:
Development of PHB (Poly-Β-Hydroxybutyrate)
Vol.I (3), Issue.I 211
Article No : 10030
Number of Downloads : 103
References :
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Barichello, J.M., Morishita, M., Takayama, K and Nagai, T. (1999). Encapsulation of
hydrophilic and lipophilic drugs in PLGA nano particles by the nano precipitation method. Drug Dev. Ind. Pharm., 25:471- 6.
Bindschaedler, C. and Toyomi, S. (1988).... More
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... Less
- Allemann, E., Leroux, J.C and Gurny, R. (1998). Polymeric nano-micro particles for the oral delivery of peptides and peptidomimetics. Adv. Drug. Deliv. Rev.,34:171- 89.
- Barichello, J.M., Morishita, M., Takayama, K and Nagai, T. (1999). Encapsulation of
- hydrophilic and lipophilic drugs in PLGA nano particles by the nano precipitation method. Drug Dev. Ind. Pharm., 25:471- 6.
- Bindschaedler, C. and Toyomi, S. (1988). Microencapsulation process and products. 35: 755-774.
- Burdon, K.L., Stokes, J.C. and Kimbrough, C.E. (1942). Studies of the common aerobic sporeforming Bacilli. Journal of bacteriology, 44 (2):163-168.
- Catarina, P. R., Ronald, J. and Neufeld, A. (2006) Methods for preparation of drugloaded polymeric nano particles 8-21.
- Ekman, B. and Sjfholm, I. (1978). Improved stability of proteins immobilized in micro particles prepared by modified emulsion polymerization technique. J Pharm Sci., 67:693 - 6.
- Fessi, H., Puisieux, F., Devissaguet, J.P., Ammoury, N. and Benita, S. (1989) Nano capsule formation by interfacial deposition following solvent displacement. Int. J. Pharm., 55: R1- R4.
- Hill, R. R. and Rendell, D. A. E. (1972). Interpretation of Infrared Spectra Heyden New York.
- Hans, M.L. and Lowman, A.M. (2002). Biodegradable nano particles for drug delivery and targeting. Current Opinion Solid State Mater Sci., 6: 319-27.
- Hartman, T.L. (1940). The use of Sudan black B as a bacterial fat stain. Stain technology, 15:23-28.
- Ishizaki, A. and Tanaka, K. (1991). Production of poly-β-hydroxybutyric acid from carbon dioxide by Alcaligenes eutrophus ATCC 17697. Journal of fermentation and bioengineering., 71: 254-257.
- John, R.D. (1987). Applications of Absorption spectroscopy of Organic Compounds. Prentice Hall of India Pvt Ltd, New Delhi.
- Li XQ, Tan, A., Voegtline, M., Bekele, S., Chen, C.S. and Aroian, R.V. 2008.Expression of Cry5B protein from Bacillus thuringiensis in plant roots confers resistance to root-knot nematode. Biol. Control., 47: 97-102.
- Pillai, O. and Panchagnula, R. (2001). Polymers in drug delivery. Curr. Opin.Chem. Biol., 34: 447451.
- Reis, C.P., Neufeld, R.J., Ribeiro, A.J and Viega, F. (2005). Insulinalginate nanospheres: influence of calcium on polymer matrix properties. Proceedings of the 13th International Workshop on Bio encapsulation. Kingston, Ontario, Canada:QueenTs University.
- Williamson, D.H. and Wilkinson, J.F. (1958). The isolation and estimation of PHB inclusions of Bacillus species. Journal of General Microbiology., 19: 198-209.
- Xiong Y.C, Yao Y.C, Zhan X.C and Chen, G.Q. (2010). Application of polyhydroxyalkanoates Nanoparticles as Intracellular Sustained Drug Release Vectors. Journal of Biomedicals Science., 21:127-140.
- Yilmaz, M., Soran, H. and Beyatli, Y. (2005). Determination of Poly-β- hydroxybutyrate (PHB) production by some Bacillus spp. World Journal of Microbiology and Biotechnology., 21: 565-566.