Induced Breeding of African Mud Catfish, Clarias gariepinus (Burchell 1822), using Different Doses of Normal Saline Diluted Ovaprim

Volume : Olumuji OK and Mustapha MK, Issue : 205, September - 2018

Abstract : African mud catfish, Clarias gariepinus is the most sought after farmed fish species in West Africa whose commercial breeding is done using expensive synthetic hormones one of which is ovaprim. The aim of the present paper is to reduce the cost of ovaprim by its dilution with normal saline in induced breeding of C. gariepinus. Induced breeding performance of Clarias gariepinus was evaluated using five different doses of normal saline diluted ovaprim at 0%,25%,75% and 100%while undiluted ovaprim served as the control. The ovaprim was administered at the rate of 0.5 ml for each treatment per kg body weight of the fish, represented as treatments A, B, C, D and E respectively. Mean weight of stripped eggs collected were 18.45 g, 17.50 g and 17.25 g in treatments A, B and C respectively with no significant difference (p<0.05) in the values. Spawning did not occur in D and E, thus no egg was collected. Percentage fertilization of the stripped eggs in treatments A, B and C were 88.70%, 87.50% and 77.38% respectively with treatment A showing significant difference (p<0.05) from B and C. Percentage hatchability from the stripped eggs were 56.58%, 54.07% and 57.75% for treatments A, B and C respectively with no significant difference (p<0.05) among the three treatments, while percentage survival of the fry were observed to be 40.27%, 40.87% and 42.52% in treatment A, B and C. There was no significant difference (p<0.05) in the survival rate among the treatments. Comparative cost benefit analysis between the control (undiluted ovaprim) and the different doses of normal saline diluted ovaprim shows that normal saline diluted ovaprim at 50% is the most cost effective. In conclusion, generic ovaprim with 50% normal saline will ensure spawning in Clarias gariepinus with high percentage hatchability of the eggs and survival of the fry.

Article: Download PDF Journal DOI : 301/704

Cite This Article:

Article No : 10066

Number of Downloads : 101

References :

1. Oladosu GA, Ayinla OA, Adeyemo AA, Yakubu AF, Ajani AA (1993) Comparative study of reproductive capacity of the African catfish species Heterobranchus bidorsalis,Clarias gariepinus and their hybrid. African Regional Aquaculture Centre Tech 92: 1-5.
2. Shepherd CJ, Bromage NR (1988) Intensive fish farming. Blackwell science Ltd., 404.
3. Viveen WJAR, Ritcher CJJ, Van Oordt PGWJ, Janseen JAL, Huisman EA (1985) Practical manual for the culture of the African Catfish Clarias gariepinus. Section for Research and Development Cooperation, the Hague, Netherlands,121.
4. Madu CT (2006) The effects of brood stock size on the economy of catfish (Clarias anguillaris) fry production using the hormone induced natural breeding technique. J Aquat Sci 21: 19-22.
5. Nwokoye CO, Nwuba LA, Eyo JE (2007) Induced propagation of African Clariid Catfish Heterbranchus bidorsalis (Gerffrey Saint Hillaries, 1809) using synthetic and homoplastic hormones. African Journal of Biotechnology 6: 2687-2693.
6. Salami AA, Balogun AM, Fagbemi S, Edibite L (1993) Utilization of Non-piscine pituitary extract in the breeding of Clarias gariepinus. Proceedings of the 10th Annual Conference of Fisheries Society of Nigeria (FISON), UNAAB, Abeokuta, Nigeria.
7. Fagbenro OA, Salami AA, Sydenham DHJ (1993) nduced Ovulation and Spawning in the Catfish, Clarias isheriensis, Using Pituitary Extracts from Non-piscine Sources. Journal of Applied Aquaculture 4: 15-20.
8. Olubiyi OA, Ayinia OA, Ayinia OA, Adeyemo AA (2005) The effects of various doses of ovaprim on reproductive performance of the African catfish Clarias gariepinus (Burchell)  and  Heterobranchus  longifilis  (Valenciennes). African Journal of Applied Zoology and Environmental Biology 7: 101-105.
9. Sahoo SK, Giri SS, Chandra S (2008) Induced spawning of Clarias batrachus (Linn): effect of ovaprim doses and latency periods on the weight of stripped eggs and ovary. Asian fisheries science 21: 333-338.
10. Madu CT (1989) Hatchery management of the Mudfish, Clarias anguillaris (L).PhD Thesis University of Jos, Nigeria, 215.
11. 1 Ayinla OA, Kayode O, Idoniboye-Obu TIE, Oresegun A, Adindu VE (1994) Use of tadpole meal as substitute for fishmeal in diet of Heterobranchus bidorsalis (Geofrey St. Hillaire, 1809). Journal of Aquaculture in the Tropics 9: 25-33.
12. Adebayo OT, Popoola OM (2008) Storage Period; its effect on efficacy of non- piscine (frog) Hormone used in inducing ovulation in African Catfish (Clarias gariepinus Burchell, 1822). International Journal of Zoological Research 4:124-128.
13. Nwadukwe FO (1993) Inducing oocyte maturation, ovulation and spawning in the African catfish, Heterobranchus longifilis Valenciennes (Pisces: Clariidae), using frog pituitary extract. Aquaculture Research 24: 625-630.
14. Odunze FC (2004) Environmentally induced physiological responses that determined fish survival and distribution: A review: 19th conference proceedings of Fisheries Society of Nigeria Ilorin, Nigeria, 429 – 435.